Fig. 2

GST-PbMAP1 specifically bound to heparin. A Heparin-binding activity was detected using western blotting with anti-GST antibody. GST-PbMAP1 could bind heparin-Sepharose but not Sepharose. GST could not bind heparin-Sepharose. B Competitive inhibition was detected using western blotting with an anti-GST antibody. As the heparin concentration increased, GST-PbMAP1 binding with heparin-Sepharose gradually decreased. C As the CSA concentration increased, no significant changes in GST-PbMAP1 binding with heparin-Sepharose were noted