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Fig. 3 | Parasites & Vectors

Fig. 3

From: A heparin-binding protein of Plasmodium berghei is associated with merozoite invasion of erythrocytes

Fig. 3

GST-PbMAP1 binds to mouse RBCs. A Indirect immunofluorescence assay using an anti-GST antibody as the primary antibody and Alexa Fluor 488 Goat anti-mouse IgG (H+L) as the secondary antibody shows the binding of GST-PbMAP1 to RBCs. GST was used as the control. Scale bar, 5 μm. B Western blotting using an anti-GST antibody shows the binding of GST-PbMAP1 to RBCs. C Cell flow cytometry analysis of erythrocyte binding by the GST-PbMAP1 with GST as a control. A clear shift is observed with GST-PbMAP1-bound erythrocytes. D The fluorescence intensity of erythrocytes bound with GST- PbMAP1 compared to that with GST and blank controls

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Parasites & Vectors

ISSN: 1756-3305

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