Fig. 4

PbMAP1 is expressed in merozoites. A Western blotting of native PbMAP1 expressed in Plasmodium berghei merozoite detected using PbMAP1-specific antibodies. B Indirect immunofluorescence of PbMAP1. Free merozoites and parasite at ring-, trophozoite-, and schizont stages were fixed, and PbMAP1 expression was detected with anti-PbMAP1 IgG (green). Parasite nuclei were stained with DAPI (blue). C Immune electron microscopy images of PbMAP1. The gold particles were localized to the merozoite membrane. Scale bar, 500 nm