Fig. 7

Construction of the PbMAP1-complemented strain (ReΔPbMAP1). A Scheme of the transfection plasmid used to target and re-introduce PbMAP1 gene in ΔPbMAP1 using the CRISPR/Cas9 method. B PCR validation of PbMAP1 reintroduction. PCR products from 5′-UTR, 3′-UTR, and PbMAP1 showed that PbMAP1 was re-introduced. C Western blotting of PbMAP1 expression in ΔPbMAP1 and ReΔPbMAP1. No protein band with expected molecular weight was detected in the ΔPbMAP1 strain, but in the ReΔPbMAP1 strain; GAPDH was used as the control. D IFA using rat anti-PbMAP1 IgG to detect PbMAP1 expression. PbMAP1 was detected in WT and ReΔPbMAP1 but not in the ΔPbMAP1 strain