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Fig. 3 | Parasites & Vectors

Fig. 3

From: Elicitation of T-cell-derived IFN-γ-dependent immunity by highly conserved Plasmodium ovale curtisi Duffy binding protein domain region II (PocDBP-RII)

Fig. 3

Copy number variation in PocDBP-RII gene. A Standard curve of qPCR for detection of PocDBP-RII and PocFBPA to calculate relative amplification efficiency (Effv%). The horizontal axis represents the Lg of DNA molecules added to the qPCR reaction system at each dilution, and the vertical axis is Ct values. The absolute value of the slope was close to zero (m = 0.0632), confirming that the amplification efficiencies of PocDBP-RII and PocFBPA were similar. B Plots of the Lg of DNA molecules versus N-fold copy number were made to reflect the relative efficiency and PocDBP-RII copy number in the two reference plasmids. C Scatter plots depicting the estimation of PocDBP-RII N-fold copy number in Plasmodium ovale curtisi isolates from different regions of Africa (n = 74)

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