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Fig. 4 | Parasites & Vectors

Fig. 4

From: Elicitation of T-cell-derived IFN-γ-dependent immunity by highly conserved Plasmodium ovale curtisi Duffy binding protein domain region II (PocDBP-RII)

Fig. 4

Expression and purification of rPocDBP-RII and detection of anti-rPocDBP-RII antibody in sera of immunized mice. A Polymerase chain reaction (PCR) amplification for PocDBP-RII (arrowhead: a 975-bp product). M: marker; lane 1: no template control; lane 2: DNA extracted from the plasma of parasite-infected patient. B SDS-PAGE analysis of rPocDBP-RII (arrowhead: a 39-kDa band) after induction with IPTG. M: protein marker; lane 1: cell homogenates of E. coli Rosetta/pET28a-PocDBP-RII without IPTG induction; lane 2: cell homogenates after IPTG induction for 6 h. C Coomassie blue-stained SDS-PAGE gel of eluted, purified and concentrated rPocDBP-RII before and after treatment with dithiothreitol (DTT). M: protein marker; lane 1: refolded rPocDBP-RII (DTT-); lane 2: denatured rPocDBP-RII (DTT +) which migrated as a single banding pattern on SDS-PAGE at the expected size of 39 kDa. D Western blot analysis of rPocDBP-RII using an anti-HIS rabbit monoclonal antibody (lane 1) or antibodies from the serum of mice immunized with rPocDBP-RII (lane 2) or PBS (lane 3)

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Parasites & Vectors

ISSN: 1756-3305

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