Fig. 6

IFN-γ/IL-10-producing T cell responses in rPocDBP-RII‑immunized mice. A Flow cytometry gating strategy for IFN-γ/IL-10-producing T cells upon rPocDBP-RII antigen. B Lymphoproliferative activity of splenocytes isolated from experimental group and PBS group, concanavalin A (Con A) worked as a positive control. Measurement of IFN-γ-producing C and IL-10-producing D CD4⁺ and CD8⁺ T cell levels in mice by flow cytometry. The data are shown as the mean ± SD. P values were calculated using Student’s t‑test. Significant differences between groups are denoted on the graph: * P < 0.05, nonsignificant (ns) differences are indicated (P > 0.05)