Table 1 List of primers used in this study
Name | Sequence (5’ → 3’) | Length (bp) | Function |
|---|---|---|---|
PocDBP 1F | GCTGATAAATTTGTTGTTAGGTCTGA | 26 | sequencing |
PocDBP S1F | GCAATGCTATTCCCAATTAAAG | 22 | |
PocDBP 1R | CCTTGACTGTTTAAATTTAAAGCATTT | 27 | |
PocDBP S1R | CTTTAATTGGGAATAGCATTGC | 22 | |
PocDBP-Q2’-F | ACACTGCGAAAATAATGGACTGCT | 24 | Construct reference plasmid with one copy of PocDBP-RII |
PocDBP-Q2’-R2 | CCATAATTAGGAACATCTCTAAAAGC | 26 | |
PocFBPA-Q1’-EcoRI-F | catgattacgaattcATGCAGGCTCCTGGTTCAGAAT | 37 | |
PocFBPA-Q1’-BamHI-R2 | atctctagaggatccTTGTGTGTGCCCCATCTGCT | 35 | |
RII/FBPA-18T-LEFT | AACCCTGGCGTTACCCAA | 18 | Construct reference plasmid with two copies of PocDBP-RII |
RII/FBPA-18T-RIGHT | TTCCCAGTCACGACGTTG | 18 | |
RII-LEFT-F | ttgggtaacgccagggttACACTGCGAAAATAATGGACTGCT | 42 | |
RII-RIGHT-R | caacgtcgtgactgggaaCCATAATTAGGAACATCTCTAAAAGC | 44 | |
PocDBP-Q2-F | AGTGACAACGATCCGAACAA | 20 | qPCR |
PocDBP-Q2-R | ACTCAACTCTTCATCATCTGCT | 22 | |
PocFBPA-Q1-F | GCAGGCTCCTGGTTCAGAAT | 20 | |
PocFBPA-Q1-R | GTCTGCGTAGATTCGTCAGC | 20 | |
PocDBP-RII-BamHI-F | tgggtcgcggatccAATATTACAAACAATGATGTAAATTATGT | 44 | Recombinant protein expression |
PocDBP-RII-XhoI-R | gtggtggtgctcgagTTTTATTCCTTTCTGCGCG | 34 |