Reassessing Stephanofilaria stilesi dermatitis in cattle, with characterization of molecular markers for confirming diagnosis

Table 1 Primers utilized to amplify Stephanofilaria stilesi DNA along with annealing temperatures and average base pair sizes

Primer	Sequences	Size (base pairs)	Tm ℃	Successful PCR	References
cox1	Forward: 5ʹ-TGA TTG GTG GTT TTG GTA A-3ʹ	~ 700 bp	52 °C	Yes	Casiraghi et al. [44]
cox1	Reverse: 5ʹ-ATA AGT ACG AGT ATC AAT ATC-3ʹ	~ 700 bp	52 °C	Yes	Casiraghi et al. [44]
12S rDNA	Forward: 5ʹ-TCG GCT ATG CGT TTT AAT TTT-3ʹ	~ 533 bp	50 °C	Yes	Casiraghi et al. [44]
12S rDNA	Reverse: 5ʹ-CAA CTT ACG CCC CTT TAG GC-3ʹ	~ 533 bp	50 °C	Yes	Casiraghi et al. [44]
18S rDNA	Forward: 5ʹ-ACC GCC CTA GTT CTG ACC GTA AA-3ʹ	~ 740 bp	58 °C	Yes	Lefoulon et al. [45]
18S rDNA	Reverse: 5ʹ-GGT TCA AGC CAC TGC GAT TAA AGC-3ʹ	~ 740 bp	58 °C	Yes	Lefoulon et al. [45]
28S rDNA	Forward: 5ʹ-CCTCAACTCAGTCGTGATTACC-3ʹ	~ 1150 bp	61.5 °C	Yes	Lefoulon et al. [45]
28S rDNA	Reverse: 5ʹ-CTCTGGCTTCATCCTGCTCA-3ʹ	~ 1150 bp	61.5 °C	Yes	Lefoulon et al. [45]
MyoHC	Forward: 5ʹ-GCA TCA RGA AGA AAT TAA TCG-3ʹ	~ 785 bp	59 °C	No	Lefoulon et al. [45]
MyoHC	Reverse: 5ʹ-GCT TCA ATT TCY TCC TCC AT-3ʹ	~ 785 bp	59 °C	No	Lefoulon et al. [45]
rbp1	Forward: 5ʹ-ACT GCA AAY ACW GCW ATT TA-3ʹ	~ 640 bp	53 °C	No	Lefoulon et al. [45]
rbp1	Reverse: 5ʹ-ACR TGA TTC ATT TCR CGT TC-3ʹ	~ 640 bp	53 °C	No	Lefoulon et al. [45]
hsp70	Forward: 5ʹ-TCR GAT TTC TTY TCT GGY A-3ʹ	~ 610 bp	55 °C	No	Lefoulon et al. [45]
hsp70	Reverse: 5ʹ-GTY TGY TTC ATA TTG AAY GC-3ʹ	~ 610 bp	55 °C	No	Lefoulon et al. [45]
nad5	Forward: 5ʹ-TTG GTT GCC TAA GGC TAT GG-3ʹ	~ 470 bp	50 °C	No	Morales-Hojas et al. [46]
nad5	Reverse: 5ʹ-CCC CTA GTA AAC AAC AAA CCA CA-3ʹ	~ 470 bp	50 °C	No	Morales-Hojas et al. [46]

ISSN: 1756-3305