Reassessing Stephanofilaria stilesi dermatitis in cattle, with characterization of molecular markers for confirming diagnosis

Table 2 Retrospective stephanofilariasis case screening with cox1 primers

Stephanofilaria histology cases	Total cases (N = 22)	Triplicates (N = 66)	Cases with Stephanofilaria stilesi sequence	Percentage	All replicates with Stephanofilaria stilesi sequence	Percentage
Adult worm present	13	39	5/13	38	7/39	18
Adult worm absent	9	27	1/9	11	1/27	4
L1 present	5	15	3/5	60	4/15	27
L1 absent	17	51	3/17	18	4/51	8
Adult + L1 present	5	15	3/5	60	4/15	27
Adult + L1 absent	9	27	1/9	11	1/27	4
Adult or L1 present	8	24	2/8	25	3/24	13

Cases were considered microscopically positive or negative depending on finding of adult worm or first-stage larvae (L1). Three random tissue samples were dissected from each case at the characteristic lesions caused by S. stilesi and subjected to DNA extraction, PCR with cox1 primers, gel electrophoresis, and Sanger sequencing

ISSN: 1756-3305