Fig. 2

Four-quadrant apoptosis diagrams of Cryptocaryon irritans tomonts treated with honokiol at various concentrations for 8 h. The C. irritans tomonts were treated with honokiol at 0.0, 0.6, 1.0, 2.0, 4.0, and 8.0 μg/ml for 8 h. The treated tomonts were incubated with Annexin V-FITC and a PI probe and analyzed with a fluorescence microplate reader (BioTek Synergy H1, BioTek Instruments, Inc., USA). The results show that with the increase in honokiol concentration, the PADR began to increase at 0.6 μg/ml and reached the highest level at 1.0 μg/ml, while the AADR and NER began to increase at 2.0 μg/ml