Fig. 3
From: Honokiol induces apoptosis-like death in Cryptocaryon irritans Tomont
Effects of the 8 h treatment of honokiol at various concentrations on the [Ca2+]i concentration, ΔΨm, ROS, QDF, and caspase-3/8/9 activities in Cryptocaryon irritans tomonts. The C. irritans tomonts were treated with honokiol at 0.0, 0.6, 1.0, 2.0, 4.0, and 8.0 μg/ml for 8 h, and the [Ca2+]i concentration, ΔΨm, ROS, QDF, and caspase-3/8/9 activities were determined. The results show that the [Ca2+]i concentration, ΔΨm, ROS, and the caspase-3/9 activities all reached the highest significant levels when the honokiol was 1.0 μg/ml, and the QDF reached the highest significant level when the honokiol was 2.0 μg/ml. a Effect of honokiol on [Ca2+]i concentration in C. irritans tomonts. b Effect of honokiol on ΔΨm in C. irritans tomonts. c Effect of honokiol on ROS in C. irritans tomonts. d Effect of honokiol on QDF in C. irritans tomonts. e Effect of honokiol on caspase-3/8/9 activities in C. irritans tomonts. The results are expressed as mean ± SD, n = 5. *Significant difference from the control sample (0.0 μg/ml), P < 0.05. **Highly significant difference from the control sample (0.0 μg/ml), P < 0.01