Table 1 Primer pairs for PCR amplification and their positions in the Eimeria zuernii mitogenome
Primer name | Location based on E. zuernii mitogenome | Primer sequence (5′ to 3′) |
|---|---|---|
Ez-1 | 1–20 | Forward: GATAAAGTCGCAGCAGTAGC |
|  | 1648–1672 | Reverse: AGGTTGGAGAGAGTGACATTAGAGA |
Ez-2 | 934–950 | Forward: TATTCCAAGTAAAGCTG |
|  | 2892–2912 | Reverse: AACCCAGCTCACGTATCACAT |
Ez-3 | 2835–2850 | Forward: GCTCATCACACCCTTG |
|  | 5090–5112 | Reverse: CTTAACCCAGCTCACGTATCACA |
Ez-4 | 5039–5060 | Forward: AAGACAGAATAGTATTTTTTAA |
|  | 511–532 | Reverse: AGTAGCACCCCAGAAGCTCATT |