Fig. 1

Ss-NOBP-1 displays conserved features with homologs from selected species. a The structures of the nobp-1 genes of Strongyloides stercoralis, Caenorhabditis elegans and Haemonchus contortus. b Alignment of the amino acid sequences of S. stercoralis NOBP-1 with homologs from eight other species. The eight selected species are Loa loa (XP_020302808.1), Brugia malayi (XP_001899836.1), Ascaris suum (GS_18771), C. elegans (NP_491090.1), Homo sapiens (NP_054781.1), Xenopus tropicalis (NP_001016830.1), Danio rerio (XP_683754.2), Drosophila melanogaster (NP_572603.1). Functional domains including PIN domain (pink), flexible linker (yellow) and zinc ribbon domain (blue) are highlighted, and the conserved aspartate residues (black) and cysteine residues (red) are marked with solid wireframes. The blue box and green arrows above the sequence represent the α-helices and β-strands. c The phylogenetic tree of S. stercoralis NOBP-1 with homologues from 10 selected species. These species contain four nematode species, one insect, two fish and amphibian species and three mammalian species. The Nob1 from Saccharomyces cerevisiae (NP_014699.1) is used as the outgroup. Accession numbers for the sequences listed follow the species name. Bootstrap values are displayed in the tree