Fig. 2

Anatomic expression pattern of Ss-NOBP-1 in Strongyloides stercoralis. a Transcriptional profiles of Ss-nobp-1 in S. stercoralis. Seven developmental stages examined were: infectious third-stage larvae (iL3), in vivo activated third-stage larvae (L3+), parasitic females (P Female), post-parasitic first-stage larvae (PP L1), post-parasitic third-stage larvae (PP L3), free-living females (FL Female) and post free-living first-stage larvae (PFL L1). Transcript abundances are expressed as fragments per kilobase of coding exon per million mapped reads (FPKM). * and ** indicate P < 0.01 and P < 0.001. b Differential interference contrast (DIC) and fluorescence (GFP) images showing the expression of GFP under the putative Ss-nobp-1 promoter in transgenic S. stercoralis larvae in the intestine. I, intestine; P, pharynx; M, mouth. Scale bar = 50 μm