Fig. 4

Determination of Schistosoma japonicum erythroid Krüppel-like factor (SjEKLF)-specific antibodies in host serum by ELISA. a BABL/c mice and b New Zealand white rabbits were percutaneously infected with cercariae (40 ± 2 parasites per mouse and 1000 ± 100 parasites per rabbit). Sera from infected animals were collected at 0, 7, 14, 21, 28, 35, 42 and 56 days post infection (dpi). Antibody titres against SjEKLF in these sera were determined by ELISA with1 μg/ml rSjEKLF. Linear charts show the detailed dynamics of antibodies against SjEKLF. *, # and Δ indicate comparisons at 0, 28 and 42 dpi, respectively. Δ indicates P < 0.05; ***, ### and ΔΔΔ indicate P < 0.0001. M, mouse; R, rabbit. c SjEKLF and d SjSP-13-specific antibodies in human serum samples were also determined by ELISA. Serum samples from patients with confirmed schistosomiasis japonica before (Sj) and 3 months after praziquantel treatment (Sj-3 M) (n = 20 each group), with confirmed echinococcosis (Es) (n = 15) and healthy individuals (healthy) (n = 20) were included in the assays. Cutoff value of positive tests was set at ≥ 2.1 times the mean optical density (OD) value of healthy individuals (dotted lines). Red and black dots indicate a positive and negative individual, respectively