Fig. 2

EgCF inhibited the secretion of inflammatory factors in macrophages. RAW 264.7 cells were treated with EgCF (50% v/v) and LPS (1 μg/ml) for 24 h. Secretion of IL-6 (A) and TNF-α (B) in cell culture supernatants was measured by ELISA. THP-1 cells were treated with EgCF (50% v/v) and LPS (1 μg/ml) for 24 h. Secretion of IL-6 (C) and TNF-α (D) in cell culture supernatants was measured by ELISA. Data are presented as the mean + SEM of three independent experiments and compared using the one-way ANOVA and Tukey test. Asterisks indicate a significant difference at **P < 0.01 and ***P < 0.001