Fig. 6

Inhibiting KDM5B attenuated the anti-inflammatory response of EgCF. RAW 264.7 cells were stimulated with EgCF (50% v/v) and LPS (1 μg/ml) for 6 h after pretreatment with CPI-455 (25 μM) for 48 h. Protein levels of KDM5B were measured by Western blotting (A). TNF-α and IL-6 mRNA expression levels (B, C) were measured by qRT-PCR. THP-1 cells pretreated with CPI-455 (25 μM) for 48 h were stimulated with EgCF (50% v/v) and LPS (1 μg/ml) for 6 h. Protein levels of KDM5B were measured by Western blotting (D). TNF-α and IL-6 mRNA expression levels (E, F) were measured by qRT-PCR. Working model for Echinococcus granulosus cyst fluid inhibiting the expression of inflammatory factors in macrophages (G). B, C, E, F Data are presented as the mean + SEM of three replicates, representing one of three independent experiments, and compared using the one-way ANOVA and Tukey test. Asterisks indicate a significant difference at *P < 0.05, **P < 0.01, and ***P < 0.001