Table 1 Primer sequences for qRT-PCR and ChIP assay
Symbol | Forward | Reverse |
|---|---|---|
Mouse TNF-α | TGATCCGCGACGTGGAA | ACCGCCTGGAGTTCTGGAA |
Mouse IL-6 | GAGGATACCACTCCCAACAGACC | AAGTGCATCATCGTTGTTCATACA |
Mouse KDM5B | ATCGCTTGCTGCACCGTTAT | CGCTCATCATCTGGCAACAG |
Mouse KDM6B | ATAGGGCCCTTCGCTATGGA | CGTTTGTGCTCAAGGTGCAG |
Mouse KDM7A | TGCAGCTCTACACGGCTCT | CCAGCTTGAACAGGTTTGGAG |
Mouse IL-10 | ACCTGGTAGAAGTGATGCC | CACCTTGGTCTTGGAGCT |
Mouse β-actin | GGCTATGCTCTCCCTCACG | GAGCAACATAGCACAGCTTCTCTTT |
Human IL-6 | GATGGATGCTTCCAATCTGGAT | AGTTCTCCATAGAGAACAACATA |
HumanKDM5B | AGTGGGCTCACATATCAGAGG | CAAACACCTTAGGCTGTCTCC |
Human TNF-α | TTCCCCAGGGACCTCTCTCTAATC | GAGGGTTTGCTACAACATGGGCTAC |
Human IL-10 | GACTTTAAGGGTTACCTGGGTTG | TCACATGCGCCTTGATGTCTG |
Human β-actin | GAGAAAATCTGGCACCACACC | GGATAGCACAGCCTGGATAGCAA |
TNF-α promoter | CAGCCACTGCTTGGCTAGAC | CGGATCCCATGGACCAACTG |
IL-6 promoter | AGGAGTGTGAGGCAGAGAGC | GTCTCCTCTCCGGACTTGTG |