Fig. 1

Role of VSPAS7 in Giardia-induced NF-κB and MAPK signaling pathways. Western blot analysis (a) and relative gray values (b) of VSPAS7 expression were performed in mouse PMs transfected with the pcDNA3.1-vspas7 vector at different times after transfection (30 h, 36 h, 42 h and 48 h). Mouse PMs pre-transfected with pcDNA3.1-vspas7 vector for 24 h were incubated with Giardia trophozoites (MOI = 1:1) for 1 h; then, VSPAS7 expression level (c) and NF-κB p65 and IκBα phosphorylation levels were detected by western blot (d) and relative gray values (e, f). Similarly, mouse PMs pre-transfected with pcDNA3.1-vspas7 vector for 24 h were incubated with Giardia trophozoites (MOI = 1:1) for 3 h, following which p38 and ERK phosphorylation levels were analyzed by western blot (g) and relative gray values (h, i). C: Control. Results are expressed as mean ± SD from three separate experiments. ns, no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001