Fig. 6

Interaction of mouse NLRP3 and Giardia VSPAS7. a Co-immunoprecipitation assays of NLRP3 and VSPAS7. HEK293T cells were transfected with pcDNA3.1-N-Flag-nlrp3 and pcDNA3.1-His-vspas7 for 24 h. The cellular proteins, anti-His/Flag tag antibody and protein A/G magnetic beads were co-incubated and analyzed by western blot. IgG: A negative control; the cellular proteins co-transfected with pcDNA3.1-N-Flag-nlrp3 and pcDNA3.1-His-vspas7, anti-IgG antibody and protein A/G magnetic beads were co-incubated. b Bimolecular fluorescence complementation experiments of mouse NLRP3 and Giardia VSPAS7. HEK293T cells were co-transfected with constructed pBiFC-nlrp3VC155 and pBiFC-vspas7VN173 plasmids for 24 h and observed by laser confocal fluorescence microscopy. The co-transfection of pBiFC-bFosVC155 and pBiFC-bJunVN173 plasmids was used as the positive control, while the co-transfection of pBiFC-VC155 and pBiFC-VN173 plasmids was used as the negative control. Blue: Hoechst 33342 staining of HEK293T cell nuclei. Green: green fluorescence emitted upon protein interactions. Scale bar: 50 μm