First isolation of Rickettsia amblyommatis from Amblyomma mixtum in Colombia

Parasites & Vectors

Table 1 List of primers used for real-time and conventional PCR

Gene	Primer name	Sequences	Size (bp)	Annealing (°C)	References
16S mitochondrial rRNA (16S mt rRNA)	16+	CCGGTCTGAACTCAGATCAAGT	472	50	Black and Piesman [22]
16S mitochondrial rRNA (16S mt rRNA)	16−	GCTCAATGATTTTTTAAATTGCTGTGG	472	50	Black and Piesman [22]
Citrate synthase (gltA)	CS5	GAGAGAAAATTATATCCAAATGTTGAT	147	60	Guedes et al. [23]; Labruna et al. [24]
	CS6	AGGGTCTTCGTGCATTTCTT
	Probe	6-FAMd (CATTGTGCCATCCAGCCTACGGT) BHQ-1 3
	CS78	GCAAGTATCGGTGAGGATGTAAT	401	48	Labruna et al. [24]
	CS323	GCTTCCTTAAAATTCAATAAATCAGGAT	401	48	Labruna et al. [24]
Outer-membrane protein B (ompB)	120-M59	CCGCAGGGTTGGTAACTGC	862	54	Roux and Raoult [25]
Outer-membrane protein B (ompB)	120-807	CCTTTTAGATTACCGCCTAA	862	54	Roux and Raoult [25]
Outer-membrane protein A (ompA)	190-70p	ATGGCGAATATTTCTCCAAAA	631	46	Regnery et al. [26]; Roux et al. [27]
Outer-membrane protein A (ompA)	190-701	GTTCCGTTAATGGCAGCATCT	631	46	Regnery et al. [26]; Roux et al. [27]
17 kDa surface antigen (htrA)	17KdsF	GCTCTTGCAACTTCTATGTT	439	56	Webb et al. [28]
17 kDa surface antigen (htrA)	17KdsR	CATTGTTCGTCAGGTTGGCG	439	56	Webb et al. [28]

ISSN: 1756-3305