Table 1 Primer sequences of the three PCRs used to detect Plasmodium and Haemoproteus parasites
From: Avian Plasmodium spp. and Haemoproteus spp. parasites in mosquitoes in Germany
Primer | Sequence | Amplicon length [bp] | Target region | Purpose of PCR | References |
|---|---|---|---|---|---|
R330F | 5′-CGTTCTTAACCCAGCTCACG-3′ | 182 | mitochondrial rDNA | Screening (real-time PCR) | [27] |
R480RL | 5′-GCCTGGAGGTWAYGTCC-3′ | ||||
H332F | 5′-GAGAATTATGGAGYGGATGGTG-3′ | 526 | cytb gene | Identification (1st nested PCR) | [27] |
HAEMNR2 | 5′-AGAGGTGTAGCATATCTATCTAC-3′ | [42] | |||
H350F | 5′-GGTGTTTTAGATATATGCATGC-3′ | 477 | cytb gene | Identification (2nd nested PCR) | [27] |
HAEMR2 | 5′-GCATTATCTGGATGTGATAATGGT-3′ | [43] |