Fig. 1From: Genetic analysis challenges the presence of Ixodes inopinatus in Central Europe: development of a multiplex PCR to distinguish I. inopinatus from I. ricinusSchematic representation of the partial TROSPA gene sequences depicting the nucleotide positions with the double peaks (small yellow bars) (KF041821 is used as a reference sequence). The chromatograms depict the forward and reverse strands of sequencing for the uncloned PCR product. 441Â cl.1 and 441Â cl.5 are sequences after cloning resolving the double peaks of hybrid ticksBack to article page