Table 1 Primers used in the RT-qPCR
Genes | Primer sequence (5′ → 3′) | Length (bp) | Accession No | |
|---|---|---|---|---|
IL-1β | F: | TTCAGGCAGGCAGTATCAC TCATTG | 169 | NM_031512.2 |
R: | ACACCAGCAGGTTATCATCATCATCC | |||
IFN-γ | F: | CTCTTCTTGGATATCTGGAGGAACTGG | 138 | NM_138880.2 |
R: | AATGACGCTTATGTTGTTGCTGATGG | |||
IL-10 | F: | TTCTTTCAAACAAAGGACCAGC | 81 | NM_012854.2 |
R: | GCAACCCAAGTAACCCTTAAAG | |||
IL-4 | F: | TACCAGGAGCCATATCCACGGATG | 139 | NM_201270.1 |
R: | TGTGGTGTTCTTCGTTGCTGTGAG | |||
TNF-α | F: | GCGACGTGGAACTGGCAGAAG | 92 | NM_012675.3 |
R: | GAATGAGAAGAGGCTGAGACATAGGC | |||
GAPDH | F: | CAAGTTCAACGGCACAGTCA | 100 | NM_017008.4 |
R: | CCATTTGATGTTAGCGGGAT | |||