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Fig. 2 | Parasites & Vectors

Fig. 2

From: Interaction of Mesonivirus and Negevirus with arboviruses and the RNAi response in Culex tarsalis-derived cells

Fig. 2

Mosquito-derived and mammalian cell lines co-infected with YicV/DeziV/DaesV. a Hsu (Culex quinquefasciatus), CT (Culex tarsalis), Aag2 (Aedes aegypti), Vero (Chlorocebus sp.) and BHK (Mesocricetus auratus) cells infected with the YicV/DeziV/DaesV mix (representative data of 2 independent repeats; except for Hsu cells). Right panel shows two additional repeats for Hsu-infected cells (Hsu-1 and Hsu-2, respectively). a, b The presence of YicV, DeziV and DaesV was detected by reverse transcription and virus-specific primers at 72 hpi (a) or at several passages for persistently infected CT and Hsu cells (b). The asterisk indicated an unspecific PCR band (verified by Sanger sequencing) in Hsu cells. DaesV, Daeseongdong virus (taxon Negevirus); DeziV, Dezidougou virus (taxon Negevirus); hpi, hours post infection; P, passage; YicV, Yichang virus (family Mesoniviridae)

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