Fig.3

Effect of YicV/DeziV/DaesV infection status on Semliki Forest or Bunyamwera orthobunya arbovirus infections in CT (Culex tarsalis)-derived cells. CT cells were infected with BUNV-NLuc (BUNV expressing NLuc; MOI 0.1) (a) or SFV6-2SG-Nluc (SFV-Nluc, SFV expressing Nluc; MOI 1 or MOI 0.1) (b, c), either single, acutely co-infected with YicV/DeziV/DaesV (YicV/DeziV/DaesV + SFV-Nluc; MOI 1, MOI 0.1 or YicV/DeziV/DaesV + BUNV-NLuc; MOI 0.1) or using cells persistently infected with YicV/DeziV/DaesV (pYicV/DeziV/DaesV + SFV-Nluc; MOI 1, MOI 0.1 or pYicV/DeziV/DaesV + BUNV-NLuc; MOI 0.1). Cells were lysed at the indicated hours post infection (hpi) and luciferase activity was measured. Results of three independent experiments performed in technical triplicates are presented. Mean values with standard error of the mean are shown. Significance was tested via unpaired t-test. BUNV infection: acute infection (24 hpi: t = 2.448, *P = 0.0263; 72 hpi: t = 2.389, *P = 0.0296); persistent infection (24 hpi: t = 5.180, ***P = 0.0002; 48 hpi: t = 9.981, ****P < 0.0001; 72 hpi: t = 11.69, ****P < 0.0001). SFV infection MOI 0.1: acute infection (24 hpi: t = 3.143, **P = 0.0063; 72 hpi: t = 10.59, ****P < 0.0001); persistent infection (18 hpi: t = 5.962, ****p < 0.0001). BUNV, Bunyamwera orthobunya virus; DaesV, Daeseongdong virus; DeziV, Dezidougou virus; MOI, multiplicity of infection; Nluc, Nano luciferase; ns, not significant; YicV, Yichang virus; SFV Semliki forest virus