Fig. 6

Production of DeziV-specific sRNAs in CT (Culex tarsalis) and Aag2 (Aedes aegypti) cells acutely infected with YicV/DeziV/DaesV or CT cells persistently infected ith YicV/DeziV/DaesV. For acute infection, cells were infected with the DaesV/DeziV/YicV mix and total RNA was isolated at 24 h post-infection. RNA of persistently infected CT cells (passage 3) were isolated, followed by β-elimination treatment (Additional file 8: Figure S4) and control (CT persistent). a The absolute frequency of sRNAs ranging in length from 18 to 31 nt that were mapped to the virus genome/antigenome. b The distribution of 21-nt-long sRNA to the indicated virus genome/antigenome. c The distribution of 26- to 30-nt-long sRNAs (piRNA-sized) to the virus genome/antigenome. Positive values (green) represent sense reads, negative values (purple) represent antisense reads. Y-scale values give the read counts, with the scale values mentioned above the graph. Representative results of the acute infection of a duplicate (Additional file 7: Figure S3). DaesV, Daeseongdong virus; DeziV, Dezidougou virus; nt, nucleotide; sRNAs, small RNAs; YicV, Yichang virus