Fig. 4

SNP analysis of DecR_SC and validation of cyt b mutations associated with decoquinate resistance. A A boxplot summary of next-generation sequencing data obtained from DecR_SC. B SNP-based PCA analysis of DecR_H, DecR_XJ and DecR_SC. C Venn diagram of genes containing non-synonymous mutations shared between DecR_H, DecR_XJ and DecR_SC. D Analysis of the transmembrane region of the cyt b gene and distribution of the non-synonymous mutations on cyt b in three drug-resistant strains. XJ-Ile63Leu is an intrinsic mutation in the XJ strain. cyt b, Cytochrome b gene; DecR, decoquinate resistance; H, Houghton strain; PCA, principal component analysis; SC, SC strain; SNP single nucleotide polymorphism; XJ, Xinjiang strain