Fig. 3

Expression levels of miR-185 and ARAF after miRNA transfection. a Quantification of miR-185 in PK-15 (porcine kidney epithelial) cells transfected with miR-185 mimic and mimic control. b Quantification of miR-185 in 3D4/21 cells (porcine alveolar macrophages) transfected with miR-185 mimic and mimic control. c Quantification of ARAF in PK-15 cells transfected with miR-185 mimic and mimic control. d Quantification of ARAF in 3D4/21 cells transfected with miR-185 mimic and mimic control. e Western blot detection of decrease in expression of ARAF protein after miRNA transfection in PK-15 cells; the bar graph indicates the relative protein expression of ARAF. f Western blot detection of decrease in ARAF protein after miRNA transfection in 3D4/21 cells; the bar graph indicates the relative protein expression of ARAF. Asterisks indicate a significant difference between cells transfected with mimic controls and cells transfected with miR-185 mimic at *P < 0.05, ***P < 0.001 and ****P < 0.0001