Fig. 4
From: Toxoplasma gondii infection regulates apoptosis of host cells via miR-185/ARAF axis
The results of the dual-luciferase reporter assay. a The binding site between miR-185 and ARAF gene. b Luciferase assay of the reporter plasmids including the wild-type (WT) and mutant (MUT) ARAF 3′-untranslated region. NC, cells transfected with psiCHECK2, and co-transfected with miR-185 mimic or mimic control, respectively; ARAF WT, cells transfected with psiCHECK2-ARAF WT, and co-transfected with miR-185 mimic or mimic control, respectively; ARAF MUT, cells transfected with psiCHECK2-ARAF MUT, and co-transfected with miR-185 mimic or mimic control, respectively. Asterisks indicate a significant difference at **P < 0.01.