Fig. 5

Apoptosis rates of different cells with different treatments. The apoptosis of cells was analysed by flow cytometry following staining with Annexin V and PI. Dot plots depict the amounts of cells stained with Annexin V and/or PI; numbers in parentheses indicate percentages of cells in each quadrant from a representative experiment. Western blot detecting the expression levels of cleaved caspase-3 in different cells with different treatments. The bar graphs indicate the apoptosis rates and relative protein expression of cleaved caspase-3. All cells were separately infected with T. gondii YZ-1 strain and control cultivation. a Apoptosis rates and the expression levels of cleaved caspase-3 in PK-15 (porcine kidney epithelial) cells transfected with miR-185 mimic or mimic control. b Apoptosis rates and the expression levels of cleaved caspase-3 in 3D4/21 cells transfected with miR-185 mimic or mimic control. Asterisk indicates a significant difference at *P < 0.05, **P < 0.01 and ***P < 0.001. PI, Propidium iodide