Fig. 6
From: Toxoplasma gondii infection regulates apoptosis of host cells via miR-185/ARAF axis
Knockout efficiency of cell lines with stable ARAF gene knockout. a Quantification of ARAF in PK-15-KOARAF cells and PK-15 cells. b Quantification of ARAF in 3D4/21-KoARAF cells and 3D4/21 cells. c Western blot detecting ARAF protein loss in PK-15-KOARAF cells; the bar graph indicates the relative protein expression of ARAF. d Western blot detecting ARAF protein loss in 3D4/21-KoARAF cells. e Mutation site of ARAF in PK-15-KOARAF and 3D4/21-KoARAF cells (adenine [A]); the bar graph indicates the relative protein expression of ARAF. Asterisks indicate a significant difference at ***P < 0.001 and ****P < 0.0001. 3D4/21-KoARAF, 3D4/21 cell line with stable ARAF gene knockout; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PK-15-KOARAF, PK-15 cell line with stable ARAF gene knockout