Fig. 8

Apoptosis rates of different cells with different treatments. The apoptosis of cells was analysed by flow cytometry following staining with Annexin V and PI. Dot plots depict the amounts of cells stained with Annexin V and/or PI; numbers in parentheses indicate percentages of cells in each quadrant from a representative experiment. Western blot detecting the expression levels of cleaved caspase-3 in different cells with different treatments. The bar graphs indicate the apoptosis rates and relative protein expression of cleaved caspase-3. All cells were separately infected with T. gondii YZ-1 strain and control cultivation. a Apoptosis rates and the expression levels of cleaved caspase-3 in PK-15 cells and PK-15-KoARAF cells. b Apoptosis rates and the expression levels of cleaved caspase-3 in 3D4/21 cells and 3D4/21-KoARAF cells. c Apoptosis rates and the expression levels of cleaved caspase-3 in PK-15-KOARAF cells transfected with the overexpression vector pcDNA3.1-Flag-ARAF or pcDNA3.1-Flag. d Apoptosis rates and the expression levels of cleaved caspase-3 of 3D4/21-KOARAF cells transfected with the overexpression vector pcDNA3.1-Flag-ARAF or pcDNA3.1-Flag. Asterisks indicate a significant difference at *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. D4/21-KoARAF, 3D4/21 cell line with stable ARAF gene knockout; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PI, propidium iodide; PK-15-KOARAF, PK-15 cell line with stable ARAF gene knockout