Table 1 Sequences of microRNA and primers used in the experiment
From: Toxoplasma gondii infection regulates apoptosis of host cells via miR-185/ARAF axis
Name | Sequences (5′ to 3′) |
|---|---|
sgRNA-F | CACCGGCCCAACAAGCAGCGCACGG |
sgRNA-R | AAACCCGTGCGCTGCTTGTTGGGCC |
Araf-F(EcoRI) | CCGGAATTCATGGAGCCACCACGGGGC |
Araf-R(XhoI) | CCGCTCGAGCTAAGGCACAAGGAGGGCT |
qPCR-Araf-F-pig | CAACACTGATGCTGCTGGTAA |
qPCR-Araf-R-pig | CAGATGGCGACTTGGAATG |
qPCR-GAPDH-F-pig | GTCGGTTGTGGATCTGACCT |
qPCR-GAPDH-R-pig | CTTGACGAAGTGGTCGTTGA |
qPCR-Araf-F-mice | CTCCACATCTACTCCTAACG |
qPCR-Araf-R-mice | CCATCACCACCTCTACCA |
qPCR-GAPDH-F-mice | TCTCCTGCGACTTCAACA |
qPCR-GAPDH-R-mice | TGTAGCCGTATTCATTGTCA |
qPCR-miR-185 | TGGAGAGAAAGGCAGTTCCTGA |
Detection-sgRNA-F | TTGACAAAGTCCTAGGCTCCAT |
Detection-sgRNA-R | CACCTTTCTGATCCACGATGT |
PsiCHECK2 (Xho I)-ARAF-F-P1 | GCCTCGAGGCCCCACCCCCAGCCACC |
Araf-M-R-P2 | GATGGAGGAGCCGGACTATTTATAAGTATCCCCAAAG |
Araf-M-R-P3 | TACTTATAAATAGTCCGGCTCCTCCATCTCCAATGGC |
PsiCHECK2 (Not I)-ARAF-F-P4 | CGGCGGCCGCCCAGCAGAATTCTGTGCTT |
miR-185 mimic | |
 Sense | UGGAGAGAAAGGCAGUUCCUGA |
 Antisense | AGGAACUGCCUUUCUCUCCAUU |
Mimic control | |
 Sense | UUCUCCGAACGUGUCACGUTT |
 Antisense | ACGUGACACGUUCGGAGAATT |