Diversity of Thelazia spp. in domestic cattle from Romania: epidemiology and molecular diagnosis by a novel multiplex PCR

Table 1 Prevalence of Thelazia spp. across the different statistical categories

Variable	Category	Sampled (n)	Positive for Thelazia spp. (n)	Prevalence of Thelazia spp. (%)	95% Confidence interval	P
Sex	Male	218	38	17.4	12.64–23.13	0.537
Sex	Female	487	98	20.1	16.8–23.91	0.537
Season	Spring	256	16	6.2	3.61–9.95	< 0.0001*
	Summer	310	62	20.0	15.93–24.81
	Autumn	100	50	50.0	39.83–60.17
	Winter	39	8	20.5	9.30–36.46
Altitude intervals (m a.s.l.)	< 200	238	48	21.0	15.88–26.81	0.016*
	≥ 200 to < 400	229	49	20.6	15.64–26.29
	≥ 400 to < 600	95	7	7.4	3.01–14.59
	≥ 600	143	32	22.4	15.84–30.1
Biogeoregion^a	P	57	14	20.9	14.13–37.76	0.518
	S	101	20	19.8	12.54–28.91
	A	164	35	20.3	15.34–28.41
	C	383	67	16.9	14.02–21.62
Ecoregion^b	PMF	350	75	24.4	17.45–26.03	< 0.0001*
	BMF	7	6	85.7	42.13–99.64
	PS	24	9	37.5	18.8–59.41
	CMCF	176	35	19.9	14.26–26.56
	EEFS	97	8	8.2	3.63–15.61
	CEMF	51	3	5.9	1.23–16.24
Total		705	136	19.3	16.55–22.37

*Statistically significant
^aBiogeregions: Pannonian (P), Steppic (S), Alpine (A) and Continental (C)
^bEcoregions: Pannonian Mixed (PMF), Balkan mixed forests (BMF), Pontic steppe (PS), Carpathian montane coniferous forests (CMCF), East European forest steppe (EEFS), Central European mixed forests (CEMF)

ISSN: 1756-3305