Fig. 5From: Identification of antigens in the Trichinella spiralis extracellular vesicles for serological detection of early stage infection in swineExpression and purification of rTs-TTPA. A The constructed vector was identified by double restriction enzyme digestion. From right to left are DNA markers, pMAL-c5X-Ts-TTPA plasmid digested with BamHI/EcoRI and pMAL-c5X-Ts-TTPA plasmid. The red arrow shows the Ts-TTPA gene. B The purified Ts-TTPA was stained with Coomassie Bright Blue. The red arrow shows the Ts-TTPA protein. C The purified rTs-TTPA was identified by monoclonal His-tag antibodyBack to article page