A novel tetra-primer ARMS-PCR approach for the molecular karyotyping of chromosomal inversion 2Ru in the main malaria vectors Anopheles gambiae and Anopheles coluzzii

Table 2 Performance of the tetra-primer amplification refractory mutation system-PCR-PCR 2Ru genotyping assay versus proven 2Ru genotyping methods (genotyping-in-thousands by sequencing and cytogenetic karyotyping

Karyotyping method	2Ru inversion genotype^a	Tetra-primer ARMS-PCR^b			Concordance %
Karyotyping method	2Ru inversion genotype^a	2R+^u/+^u	2Ru/+^u	2Ru/u	Concordance %
GT-seq	2R+^u/+^u	238	–	–	95.9
	2Ru/+^u	7	92	5
	2Ru/u	-	3	20
Cytogenetic karyotyping	2R+^u/+^u	76	–	–	100
	2Ru/+^u	–	5	–
	2Ru/u	–	–	2

ARMS Amplification refractory mutation system, GT-seq genotyping-in-thousands by sequencing
^a2R+^u/+^u, Standard (un-inverted) homozygote; 2Ru/+^u, heterozygote; 2Ru/u, inverted homozyogte
^bNumbers in italics show discordance in the results of the two methods

ISSN: 1756-3305