Table 1 Primers used in this study
Gene | Sequence (5′–3′) | Amplified gene size (bp) | GeneBank ID | Amplification temperature (℃) |
|---|---|---|---|---|
18S | F: ACCGTTTCGGCTTTTGTTGG | 196 | XR_002966730.1 | 59 |
R: CCCCGAACTACCCTCCTTCA | ||||
Bax | F: AATTGGAGATGAACTGGACAGCA | 153 | NM_007527.3 | 56 |
R: AAGTAGAAGAGGGCAACCACGCG | ||||
Bcl-2 | F: ACTTCTCTCGTCGCTACCGT | 136 | AH001858.2 | 56 |
R: ACAATCCTCCCCCAGTTCAC | ||||
Caspase-3 | F: GCAGCTTTGTGTGTGTGATTC | 136 | BC038825.1 | 56 |
R: AGTTTCGGCTTTCCAGTCAG | ||||
CD28 | F: CCCCTGCTTGTGGTAGA | 177 | NM_007642.4 | 56 |
R: GTTGAACTCGGCATTCG | ||||
CTLA-4 | F: GTGGGCTTCCTAGATTACC | 140 | NM_009843.4 | 56 |
R: ACAAAGTATGGCGGTGG | ||||
GAPDH | F: TCTTGGGCTACACTGAGGAC | 126 | GU214026.1 | 56 |
R: CATACCAGGAAATGAGCTTGA | ||||
ICOS | F: CACTGCTTCAGGGCTTAG | 133 | NM_017480.2 | 56 |
R: GGGTGGGAGGGAATGTA | ||||
PD-1 | F: TGTGCCTGGAAATGGAG | 110 | NM_008798.3 | 56 |
R: GGTGGCTTTAGGTGCTG | ||||
PD-L1 | F: GCCTCAGCACAGCAACTT | 157 | BC066841.1 | 56 |
R: CGTGATTCGCTTGTAGTCC | ||||
TCR | F: GCTGGTGGGTGAATGG | 146 | AH002089.2 | 56 |
R: GCAGCGGAAGTGGTTT | ||||
Tim-3 | F: CACCATCGAGGAGAACG | 139 | NM_134250.2 | 56 |
R: AAGGGAAATACCAAAGTCAG | ||||
TLR2 | F: TGCCACCATTTCCACG | 184 | AF185284.1 | 56 |
R: AGGGCGGGTCAGAGTT | ||||
TLR4 | F: AGCATGGACCTTACCGG | 127 | NM_021297.3 | 56 |
R: TAGCCTCTTCTCCTTCAGATT | ||||
TLR9 | F: TCTCCAACCGTATCCACC | 138 | AF348140.1 | 56 |
R: TGGGCTCAATGGTCATG |