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Fig. 1 | Parasites & Vectors

Fig. 1

From: A nanoparticle vaccine displaying the ookinete PSOP25 antigen elicits transmission-blocking antibody response against Plasmodium berghei

Fig. 1

VLP-PSOP25 vaccine design and characterization. a Schematic diagram of AP205-2*SpyTag and PSOP25-SpyCatcher coupling via spontaneous isopeptide bonds formed between SpyTag and SpyCatcher. b Purified recombinant proteins/components were separated on 10% reduced SDS-PAGE gels and stained with Coomassie blue. The left panel shows the expected ~ 24 kDa band for purified recombinant AP205-2*SpyTag. The middle panel shows the purified recombinant PSOP25-SpyCatcher with a ~ 60-kDa band. The right panel demonstrates that mixing of AP205-2*SpyTag VLP with PSOP25-SpyCatcher resulted in four different bands from top to bottom representing as: a conjugate of two PSOP25 antigens bound to each end of an AP205-2*SpyTag protein (144 kDa), a conjugate of a single PSOP25 antigen and the AP205-2*SpyTag protein (84 kDa), uncoupled PSOP25 antigen (60 kDa), and unconjugated AP205-2*SpyTag protein (24 kDa). c Images of negative staining transmission electron microscopy for the VLP-PSOP25 vaccine. The top image shows uniform, non-aggregated particles of an average size of ~ 38 nm. The image below shows typical virus-like particle shapes

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Parasites & Vectors

ISSN: 1756-3305

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