Fig. 8
Lentinan inhibited the proliferation of Toxoplasma gondii in BV2 cells in vitro. a Direct inhibition rates on tachyzoites with indicated lentinan or SD treatment. b qRT-PCR analysis of SAG1 mRNA expression in BV2 cells for the prevention experiment. c qRT-PCR analysis for SAG1 mRNA expression in BV2 cells for the therapeutic experiment. d–g For the prevention experiment, immunofluorescence staining with anti-T. gondii antibody was performed to detect the effect of 4 μg/ml lentinan or 100 μg/ml SD on intracellular and extracellular T. gondii tachyzoites numbers in BV2 cells: d Representative immunofluorescence staining of T. gondii tachyzoites, the tachyzoites were indicated in green, whereas the DAPI stain (blue) indicated the location and size of nuclei (scale bar: 20 μm). e Total number of T. gondii per field. f Total number of extracellular T. gondii per field. g Total number of intracellular T. gondii per field. h–k For the therapeutic experiment, immunofluorescence staining with anti-T. gondii antibody was performed to detect the effect of 4 μg/ml lentinan or 100 μg/ml SD on intracellular and extracellular T. gondii tachyzoite numbers in BV2 cells: h representative immunofluorescence staining of T. gondii tachyzoites, the tachyzoites are indicated in green, whereas the DAPI stain (blue) indicates the location and size of nuclei (scale bar: 20 μm). I Total number of T. gondii per field. J Total number of extracellular T. gondii per field. k Total number of intracellular T. gondii per field. Con: control group; Tg: T. gondii tachyzoite-infected group; SD sulfadiazine. Values are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001