Fig. 4

Replication kinetics of CFAV in the parental Aedes aegypti mosquitoes after IT injection. Four hundred female Ae. aegypti mosquitoes (Menghai strain) were intrathoracically injected with CFAV stock and the viral RNA and rps6 expression on the indicated days were examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) (n = 15 on each day). A The CFAV load was normalized to the host rps6 messenger RNA and the results are expressed on a log10 scale. Blue circles represent the CFAV load and the mean values are indicated by the horizontal line for each group. The CFAV infection rate (IR) is presented as a red line. B The CFAV copies per mosquito are shown as blue circles. For other abbreviations, see Figs. 1 and 3