Fig. 5

Replication kinetics of CFAV in different tissues of Aedes aegypti after IT injection. Aedes aegypti was dissected on the indicated days post-infection, and the ovary and carcass were examined for CFAV infection by RT-qPCR (n = 10 on each day). IR of CFAV in the ovary and carcass of Ae. aegypti Menghai strain (A) or Haikou strain (C). CFAV copies normalized to host rps6 mRNA in the ovary and carcass of Ae. aegypti Menghai strain (B) or Haikou strain (D). Each filled circle represents an individual sample, and only viral RNA positive values are shown. Box plots show the median and the 25-75th percentiles, and the whiskers denote the maximum and minimum values. As the residuals of the viral loads did not pass normality and heteroscedasticity tests, they were analyzed by two-tailed Mann–Whitney test. ** P < 0.01, *** P < 0.001, **** P < 0.0001