Fig. 3

Assessment of the sequence representation bias in the NGS method. Two and four preparations of a pool containing 100 L1 of each of five laboratory strains, T. spiralis (T1), T. nativa (T2), T. pseudospiralis (T4), T. murrelli (T5) and Trichinella T6, were analysed by the NGS method in two separate runs. Individual values representing the percent of reads mapped to a reference sequence are depicted as a scatter plot with median represented by a solid line and range depicted by bars. The shown p-values are exact values. Asterisks indicate statistical significance of the difference in the determined proportion of reads mapped to a reference from the actual proportion (i.e., 20%) of this genotype in a pool