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Table 1 Oligonucleotides used in this study

From: Identification of Trichinella taxa by ITS-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections

Primer name

Primer sequence (5′–3′)

ITS-1-F-Adp

TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCTGCGGAAGGATCATTATCGT

ITS-1-F-Adp1N

TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNCTGCGGAAGGATCATTATCGT

ITS-1-F-Adp2N

TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNCTGCGGAAGGATCATTATCGT

ITS-1-F-Adp3N

TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNCTGCGGAAGGATCATTATCGT

ITS-1-R-Adp

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAACCGTCATGTTGCACAAGTC

ITS-1-R-Adp1N

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNAACCGTCATGTTGCACAAGTC

ITS-1-R-Adp2N

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNAACCGTCATGTTGCACAAGTC

ITS-1-R-Adp3N

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNNAACCGTCATGTTGCACAAGTC

  1. Sequences complementary to the target region are underlined
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Parasites & Vectors

ISSN: 1756-3305

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