Fig. 2

Identification of rTsTryp. A SDS-PAGE of rTsTryp. Lanes: M, Protein marker; 1, lysates of non-induced BL21 containing the pET-32a/TsTryp plasmid; 2, lysates of induced BL21 with pET-32a/TsTryp; 3, purified rTsTryp. b Western blot of rTsTryp. Lanes: M, Protein marker; 1, lysates of non-induced pET-32a/TsTryp was not identified by serum of infected mice (murine infection serum); 2, lysates of pET-32a/TsTryp following induction were identified using murine infection serum; 3, 4, purified rTsTryp probed using murine infection serum (lane 3) and monoclonal antibody to His tag (lane 4), respectively. Arrow indicates the rTsTryp protein band of 91.6 kDa. BL21, Escherichia coli strain BL21; rTsTryp, recombinant T. spiralis trypsin; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis