Fig. 3

Antigenicity analysis of rTsTryp. a SDS-PAGE of rTsTryp. Lanes: M, Protein marker; 1, muscle larvae crude antigens; 2, muscle larvae ES antigens; 3, purified rTsTryp. b Western blot of rTsTryp. Lanes: M, Protein marker; 1, 2, 3, natural TsTryp in muscle larva crude antigens (lane 1), ES antigens (lane 2) and rTsTryp (lane 3), identified by anti-rTsTryp serum; 4–9, natural TsTryp in muscle larva crude antigens (lane 4) and ES antigens (lane 5) and rTsTryp (lane 6) probed using infection serum, but not probed using uninfected murine serum (lanes 7, 8, 9, respectively); 10, 11, 12, natural TsTryp in muscle larva crude antigens (lane 10) and ES antigens (lane 11) not recognized by anti-TRX serum, but rTsTryp was recognized by anti-TRX serum (lane 12). c Western blot of natural TsTryp in crude antigens of muscle larvae, IIL, 3 dpi adult worms, 6 dpi adult worms and NBL (lanes 1–5, respectively), identified using immune serum against rTsTryp. d Natural TsTryp in ES antigens of muscle larvae, IIL and 3 dpi adult worms (lanes 1, 2, 3, respectively) were identified using immune serum against rTsTryp by western blot. ES, Excretory/secretory; IIL, intestinal infective larvae; NBL, newborn larvae; rTsTryp, recombinant T. spiralis trypsin; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TRX, thioredoxin