Fig. 5

Expression of TsTryp in the epicuticle of diverse developmental stages of T. spiralis by IFT. Entire intact worms were incubated with immune serum against rTsTryp, and a green immunofluorescence signal was observed on the outer cuticle of T. spiralis at diverse life-cycle phases, including 6- and 12-hpi IIL, 3- and 6-dpi AW and NBL, but not ML. Pre-immune serum and anti-TRX serum did not detect any immunofluorescence signal in the worms. Trichinella-infected murine serum was applied as a positive control of reproducibility. Scale bars: 50 μm (ML, 6- and 12-hpi IIL); 100 μm (3- and 6-dpi AW); 25 μm (NBL). AW, Adult worms; IFT, immunofluorescence test; IIL, intestinal infective larvae; ML, muscle larvae; NBL, newborn larvae; rTsTryp, recombinant T. spiralis trypsin; TRX, thioredoxin