Fig. 7

Quality assessment of rTsTryp and ES antigens by western blot. ML ES antigens and rTsTryp were identified by western blot using T. spiralis-infected murine serum collected at 14 and 35 dpi, respectively. a SDS-PAGE assay of rTsTryp and ML ES antigens. Lanes: M, Protein marker; 1, ML ES antigens; 2, rTsTryp. b Western blot of ES antigens using 20 murine infection serum samples collected at 14 dpi (lanes 1–20), showing recognition of three main bands (49.1, 44.5, 40.6 kDa) of ES antigens. c Western blot of rTsTryp using 20 murine infection serum samples collected at 14 dpi (lanes 1–20), showing recognition of one distinct band of rTsTryp at 91.6 kDa. d Western blot of ES antigens using 30 murine infection serum samples collected at 35 dpi (lanes 1–30), showing recognition of four distinct bands (60, 49.1, 44.5, 40.6 kDa) of ES antigens. e Western blot of rTsTryp using 30 murine infection serum samples collected at 35 dpi (lanes 1–30), showing one distinct rTsTryp band at 91.6 kDa that was more evident than shown in c. f, g rTsTryp (f) and ML ES antigens (g) were not recognized by 30 uninfected murine serum samples. dpi, Days post-infection; ES, excretory/secretory; ML, muscle larvae; rTsTryp, recombinant T. spiralis trypsin; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis