Fig. 9

Western blot confirmation of positive serum in the ELISA test. a Western blot confirmation of positive serum at 8 dpi according to the rTsTryp-ELISA test. Seven positive serum samples (lanes 1–7) and 13 negative serum samples (lanes 8–20) in the rTsTryp-ELISA test were verified to be positive and negative, respectively, by rTsTryp-Western blot. b Western blot confirmation of positive serum at 10 dpi according to the rTsTryp-ELISA test. The 14 positive serum samples (lanes 1–14) and 6 negative serum samples (lanes 15–20) in the rTsTryp-ELISA test proved to be positive and negative, respectively, by rTsTryp-western blot. c Western blot confirmation of positive serum at 10 dpi in the ES-ELISA test. The 8 positive serum samples (lanes 1–8) and 12 negative serum samples (lanes 9–20) according to the ES-ELISA test were validated to be weakly positive and negative, respectively, by ES-western blot. All of the positive bands of rTsTryp and ES antigens recognized by western blot with early infection serum were indistinct and weak. The black arrow indicates the rTsTryp band of 91.6 kDa recognized by infection sera; the red arrow indicates the ES antigen band of 49.1 kDa identified by murine infection serum at 10 dpi. dpi, Days post-infection; ELISA, enzyme-linked immunosorbent assay; ES, excretory/secretory; M, protein marker; rTsTryp, recombinant T. spiralis trypsin